2A6P
Structure Solution to 2.2 Angstrom and Functional Characterisation of the Open Reading Frame Rv3214 from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 113 |
| Wavelength(s) | 0.91840, 0.97972 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.343, 79.844, 52.839 |
| Unit cell angles | 90.00, 109.11, 90.00 |
Refinement procedure
| Resolution | 28.900 - 2.200 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.22600 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.105 | 0.157 |
| Number of reflections | 22262 | |
| <I/σ(I)> | 57.3 | 10.1 |
| Completeness [%] | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | MPD, Lithium sulphate, Imidazole-HCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
