2A6N

Dihydrodipicolinate synthase (E. coli)- mutant R138A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	110
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	120.989, 120.989, 111.302
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.330 - 1.940
R-factor	0.16421
Rwork	0.163
R-free	0.19142
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.339
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.600	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.063	0.394
Number of reflections	87227
<I/σ(I)>		4
Completeness [%]	100.0	100
Redundancy	5.63	5.43

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10	284	protein solution (~5 mg/ml in Tris.HCl 20 mM, pH 8, 2.5 uL), precipitant (K2HPO4 1.8 M, pH 10, 1.2 uL), and N-octyl- -R-glucopyranoside (6% w/v, 0.6 uL), VAPOR DIFFUSION, HANGING DROP, temperature 284K