2A6L

Dihydrodipicolinate synthase (E. coli)- mutant R138H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	121.016, 121.016, 111.414
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.270 - 2.050
R-factor	0.184
Rwork	0.183
R-free	0.21280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.106
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.300	33.300
High resolution limit [Å]	2.050	2.050
Rmerge		0.087
Number of reflections	59240
<I/σ(I)>		10.4
Completeness [%]	99.7	99.7
Redundancy		4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	10	285	protein solution (~5 mg/ml in Tris.HCl 20 mM, pH 8, 2.5 uL), precipitant (K2HPO4 1.8 M, pH 10, 1.2 uL), and N-octyl- -R-glucopyranoside (6% w/v, 0.6 uL), VAPOR DIFFUSION, temperature 12K