2A6K
Crystal Structure Analysis of the germline antibody 36-65 Fab in complex with the dodecapeptide SLGDNLTNHNLR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.135, 144.920, 70.991 |
| Unit cell angles | 90.00, 104.35, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.246 |
| Rwork | 0.245 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.735 |
| Data reduction software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.123 | |
| Number of reflections | 19662 | |
| <I/σ(I)> | 4 | |
| Completeness [%] | 94.9 | 95.9 |
| Redundancy | 3.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 20% PEG 8000, cacodylate, azide, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
