2A6I
Crystal structure analysis of the anti-arsonate germline antibody 36-65 in complex with a phage display derived dodecapeptide KLASIPTHTSPL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.182, 76.971, 59.122 |
| Unit cell angles | 90.00, 101.97, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.246 |
| Rwork | 0.245 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.681 |
| Data reduction software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.104 | |
| Number of reflections | 15724 | |
| <I/σ(I)> | 5.1 | 1.7 |
| Completeness [%] | 94.2 | 96.3 |
| Redundancy | 2.9 | 2.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 16% PEG 8000, cacodylate, 0.05% azide , pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
