2A6D
Crystal structure analysis of the anti-arsonate germline antibody 36-65 in complex with a phage display derived dodecapeptide RLLIADPPSPRE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.302, 145.670, 71.347 |
| Unit cell angles | 90.00, 104.43, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.900 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fab |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.839 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.130 |
| Number of reflections | 23421 |
| <I/σ(I)> | 6.87 |
| Completeness [%] | 83.0 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 18% PEG 8000, cacodylate, 0.05% azide, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
