2A5Z
Crystal Structure of Protein of Unknown Function SO2946 from Shewanella oneidensis MR-1
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-29 |
| Detector | SBC-1 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.590, 53.076, 112.652 |
| Unit cell angles | 90.00, 95.66, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.015 |
| R-factor | 0.16845 |
| Rwork | 0.167 |
| R-free | 0.20274 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.158 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.120 | |
| Number of reflections | 45258 | |
| Completeness [%] | 99.6 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Peg 3350, 0.2 Mgcl2, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
