2A5L
The crystal structure of the Trp repressor binding protein WrbA from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-20 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9795001 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.927, 62.927, 190.022 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.900 - 1.700 |
| R-factor | 0.16 |
| Rwork | 0.158 |
| R-free | 0.19561 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.622 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 40802 | |
| <I/σ(I)> | 18.1 | 5.9 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 6.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 297 | 30% PEG4K. 0.2M NaAcetate, 0.1M Tris pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
