2A5J
Crystal Structure of Human RAB2B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-29 |
| Detector | SBC-3 |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.104, 54.732, 70.765 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.600 - 1.501 |
| R-factor | 0.19611 |
| Rwork | 0.195 |
| R-free | 0.22466 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oiv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.285 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.600 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.046 | 0.419 |
| Number of reflections | 27473 | |
| <I/σ(I)> | 11.8 | 2.9 |
| Completeness [%] | 97.7 | 81 |
| Redundancy | 6.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 300 | 30% PEG5000, 02M Ammonium Sulfate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, temperature 300K |
