2A4K
3-Oxoacyl-[acyl carrier protein] reductase from Thermus thermophilus TT0137
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.013, 91.565, 54.847 |
| Unit cell angles | 90.00, 104.24, 90.00 |
Refinement procedure
| Resolution | 46.078 - 2.301 |
| Rwork | 0.214 |
| R-free | 0.24780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uls |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.434 |
| Data scaling software | LSCALE |
| Phasing software | EPMR (2.5) |
| Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 53.190 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 118284 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | modified microbatch | 5.5 | 291 | 0.1M citrate, 30v/v% 1,4-butanediol, pH 5.5, modified microbatch, temperature 291K |
