2A4A
Deoxyribose-phosphate aldolase from P. yoelii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-19 |
| Detector | SBC-3 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.121, 143.472, 50.156 |
| Unit cell angles | 90.00, 106.24, 90.00 |
Refinement procedure
| Resolution | 34.680 - 1.840 |
| R-factor | 0.20503 |
| Rwork | 0.203 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jcl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.147 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.680 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.680 | 0.501 |
| Number of reflections | 41400 | |
| <I/σ(I)> | 20.3 | 1.56 |
| Completeness [%] | 98.0 | 87.2 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 298 | PEG4K, Tris pH 8.5, NaCl 0.2 M, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 8.50 |
