2A46
Crystal structures of amFP486, a cyan fluorescent protein from Anemonia majano, and variants
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-11-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.82653 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 112.541, 112.541, 81.436 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.700 - 1.650 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g7k |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR (2.5) |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.700 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.064 | 0.340 |
| Number of reflections | 31584 | |
| Completeness [%] | 99.9 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.3 | 1 M Sodium/Potassium Tartrate, 0.16 M Lithium Sulfate, 0.1 M CHES pH 9.3, VAPOR DIFFUSION, HANGING DROP, temperature 110K |
