2A46
Crystal structures of amFP486, a cyan fluorescent protein from Anemonia majano, and variants
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2003-11-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.82653 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 112.541, 112.541, 81.436 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.700 - 1.650 |
R-factor | 0.167 |
Rwork | 0.165 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g7k |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR (2.5) |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.700 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.064 | 0.340 |
Number of reflections | 31584 | |
Completeness [%] | 99.9 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.3 | 1 M Sodium/Potassium Tartrate, 0.16 M Lithium Sulfate, 0.1 M CHES pH 9.3, VAPOR DIFFUSION, HANGING DROP, temperature 110K |