2A3G
The structure of T6 bovine insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-11 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | H 3 |
| Unit cell lengths | 82.554, 82.554, 33.756 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.500 - 2.250 |
| R-factor | 0.162 |
| Rwork | 0.162 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ins |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.080 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.500 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.051 | 0.207 |
| Number of reflections | 4345 | |
| <I/σ(I)> | 18.1 | 2.81 |
| Completeness [%] | 99.5 | 94 |
| Redundancy | 4.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 298 | HCl, ZINC ACETATE, SODIUM CITRATE, ACETONE, pH 6.2, SLOW COOLING, temperature 298 K, pH 6.20 |
