2A39
HUMICOLA INSOLENS ENDOCELLULASE EGI NATIVE STRUCTURE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 270 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 102.900, 102.900, 282.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.183 * |
| Rwork | 0.180 |
| R-free | 0.22000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NATIVE TRICHODERMA REESEI CELLOBIOHYDROLASE PDB ENTRY 1CEL |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.031 |
| Data reduction software | DENZO |
| Data scaling software | Agrovata |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.069 | 0.209 |
| Total number of observations | 289090 * | |
| Number of reflections | 63790 | |
| <I/σ(I)> | 15 | 5.9 |
| Completeness [%] | 96.6 | 92.4 |
| Redundancy | 3.9 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8 | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris-HCl | 20 (mM) | |
| 2 | 1 | 1 | ammonium sulfate |
