2A2U
THE CRYSTAL STRUCTURES OF A2U-GLOBULIN AND ITS COMPLEX WITH A HYALINE DROPLET INDUCER.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source details | OTHER |
| Temperature [K] | 290 |
| Detector technology | AREA DETECTOR |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.820, 62.340, 114.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | A MONOCLINIC FORM OF A2U (REF 3) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.700 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.082 | 0.260 |
| Total number of observations | 78794 * | |
| Number of reflections | 25797 | |
| <I/σ(I)> | 13.5 | 1.9 |
| Completeness [%] | 95.1 | 94.6 |
| Redundancy | 3.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.7 | pH 4.70 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 15 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 300 (mM) | can be replaced by 500mM NaCl |
| 4 | 1 | reservoir | 50 (mM) |
