2A2N
Crystal Structure of the peptidylprolyl isomerase domain of Human PPWD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-07 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.658, 39.893, 115.638 |
| Unit cell angles | 90.00, 122.33, 90.00 |
Refinement procedure
| Resolution | 23.200 - 1.650 |
| R-factor | 0.20169 |
| Rwork | 0.199 |
| R-free | 0.24538 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x07 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.368 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.200 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.036 | |
| Number of reflections | 59800 | |
| <I/σ(I)> | 34.86 | 2 |
| Completeness [%] | 91.4 | 63.1 |
| Redundancy | 3.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 298 | 1.7M NH4SO4, 0.1M sodium cacodylate pH 5.7, 0.2M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
