2A2G

THE CRYSTAL STRUCTURES OF A2U-GLOBULIN AND ITS COMPLEX WITH A HYALINE DROPLET INDUCER.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	277
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	62.810, 98.010, 123.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.900
R-factor	0.248
Rwork	0.248
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AN ORTHORHOMBIC FORM OF A2U
RMSD bond length	0.005
RMSD bond angle	24.800 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	2.980
High resolution limit [Å]	2.900	2.900
Rmerge	0.102	0.350
Total number of observations	49636 *
Number of reflections	15350
<I/σ(I)>	11.3	3.6
Completeness [%]	88.0	91.3
Redundancy	3.2	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.8	277 *	pH 4.80

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG4000	10-20 (%)
3	1	reservoir	sodium acetate	0.1 (M)
4	1	reservoir	ammonium sulfate	0.175 (mM)