2A21
Aquifex aeolicus KDO8PS in complex with PEP, PO4, and Zn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 84.527, 84.527, 159.752 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.860 - 1.800 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.21000 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.900 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 61339 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.89 | 277 | PEG4K, Na acetate, pH 4.89, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
