2A1S
Crystal structure of native PARN nuclease domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 205.540, 123.016, 82.844 |
| Unit cell angles | 90.00, 112.59, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.21971 |
| Rwork | 0.219 |
| R-free | 0.25362 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a1r |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.334 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.079 | 0.628 |
| Number of reflections | 58173 | |
| <I/σ(I)> | 6.2 | 2 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG3350, 0.2M ammonium tartrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
