2A1L
Rat PITP-Beta Complexed to Phosphatidylcholine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 65.945, 65.948, 133.513 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.230 - 2.180 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t27 PITP-ALPHA MINUS THE REGULATORY LOOP AND THE PHOSPHOLIPID LIGAND |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.230 | 2.260 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Number of reflections | 15160 | |
| <I/σ(I)> | 39.2 | 3.8 |
| Completeness [%] | 94.3 | 80.6 |
| Redundancy | 19.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | PEG 400, SODIUM CHLORIDE, SODIUM Potassium phosphate buffer, pH 6.20, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
