2A1I
Crystal Structure of the Central Domain of Human ERCC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 116 |
| Detector technology | CCD |
| Collection date | 2004-11-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00636 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.070, 50.291, 72.216 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.20624 |
| Rwork | 0.204 |
| R-free | 0.24762 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.701 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 11932 | |
| Completeness [%] | 99.7 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | MES, PEG 5000 MME, GLYCEROL, pH 6.5, VAPOR DIFFUSION, SITTING DROP,temperature 295K |
