2A1F
Crystal Structure of Uridylate kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.369, 79.889, 79.899 |
| Unit cell angles | 94.85, 96.68, 96.88 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.21296 |
| Rwork | 0.210 |
| R-free | 0.26327 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bne |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.332 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.065 | 0.166 |
| Number of reflections | 105743 | |
| <I/σ(I)> | 8.4 | 2.88 |
| Completeness [%] | 96.4 | 88.7 |
| Redundancy | 1.7 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10% PEG4K, 0.1M Bis-Tris pH6.5, 0.6M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
