2A1D

Staphylocoagulase bound to bovine thrombin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1997-04-01
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	183.780, 102.540, 134.040
Unit cell angles	90.00, 129.14, 90.00

Refinement procedure

Resolution	31.690 - 3.500
Rwork	0.233
R-free	0.30600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HUMAN THROMBIN-STAPHYLOCOAGULASE COMPLEX
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	55.700
High resolution limit [Å]	3.400
Number of reflections	138556

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1M IMIDAZOLE, 0.2M AMMONIUM FORMATE, 12% POLYETHYLENE GLYCOL 4000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K