2A18
carboxysome shell protein ccmK4, crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.549, 125.040, 32.521 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.500 - 2.280 |
| R-factor | 0.20364 |
| Rwork | 0.203 |
| R-free | 0.22236 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a10 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.006 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.360 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Number of reflections | 13846 | |
| <I/σ(I)> | 23.2 | 8.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.6 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1M sodium citrate, pH 4.6, 30% PEG 4000, 0.2M ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
