2A13

X-ray structure of protein from Arabidopsis thaliana AT1G79260

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-13
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 21 21 2
Unit cell lengths	60.056, 80.026, 36.886
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.060 - 1.320
R-factor	0.15708
Rwork	0.156
R-free	0.18600
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.311
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.340
High resolution limit [Å]	1.310	1.310
Rmerge	0.066	0.240
Number of reflections	41019
<I/σ(I)>	14.91	6.178
Completeness [%]	96.3	93.1
Redundancy	7.5	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	10 MG/ML PROTEIN, 18% PEG2K, 0.025 M TRIS, pH 8.0, vapor diffusion, hanging drop, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	8	277	10 MG/ML PROTEIN, 18% PEG2K, 0.025 M TRIS, pH 8.0, vapor diffusion, hanging drop, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	8	277	10 MG/ML PROTEIN, 18% PEG2K, 0.025 M TRIS, pH 8.0, vapor diffusion, hanging drop, temperature 277K