2A0P

Crystal structure of RNA oligomer containing 4'-thioribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	128
Detector technology	IMAGE PLATE
Collection date	1997
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	H 3 2
Unit cell lengths	42.440, 42.440, 127.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 1.950
R-factor	0.2021
Rwork	0.202
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	259d
RMSD bond length	0.030
RMSD bond angle	3.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.950
Rmerge	0.067
Number of reflections	3077

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	Cond 39 Hampton Screen I, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K