29ZV

Crystal structure of Fc heterodimer with StrandLock CH3 interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.619923
Spacegroup name	P 21 21 21
Unit cell lengths	49.639, 75.314, 148.553
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.270 - 2.064
R-factor	0.2293
Rwork	0.227
R-free	0.27860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.695
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.270	2.100
High resolution limit [Å]	2.064	2.064
Rpim	0.039
Number of reflections	36403	1506
<I/σ(I)>	10.3
Completeness [%]	99.4
Redundancy	11.9
CC(1/2)		0.358

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M ammonium formate, pH 6.6, and 20% (w/v) polyethylene glycol (PEG) 3350