29ZH
Crystal structure of human NIF3L1 - half open hexamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97620 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 171.600, 171.600, 167.526 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.900 - 3.500 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.803 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.540 | 3.660 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.222 | 3.314 |
| Rpim | 0.072 | 1.049 |
| Number of reflections | 36362 | 4377 |
| <I/σ(I)> | 12.7 | 0.83 |
| Completeness [%] | 100.0 | |
| Redundancy | 20.1 | |
| CC(1/2) | 0.980 | 0.270 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 12 % w/v PEG 6000 0.1 M Sodium citrate 5.6 0.1 M Lithium sulfate |
