29ZG
Crystal structure of human NIF3L1 protein with hexamer in ASU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.406, 108.773, 245.034 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.200 - 3.000 |
| R-factor | 0.2069 |
| Rwork | 0.205 |
| R-free | 0.24580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.015 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 3.060 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 122851 | 6712 |
| <I/σ(I)> | 4.91 | 0.44 |
| Completeness [%] | 100.0 | |
| Redundancy | 5.3 | |
| CC(1/2) | 0.972 | 0.129 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.6 M Ammonium sulfate 0.1 M MES 6.5 |
