29ZF

Crystal structure of human NIF3L1 protein with trimer in ASU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9184
Spacegroup name	P 43 2 2
Unit cell lengths	101.011, 101.011, 276.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.870 - 2.270
R-factor	0.198
Rwork	0.196
R-free	0.24880
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	1.704
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (("2.0_5936": ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.530	2.400
High resolution limit [Å]	2.270	2.270
Rmeas	21.500	348.800
Number of reflections	67367	20042
<I/σ(I)>	8.4
Completeness [%]	99.2	98.3
Redundancy	14.65
CC(1/2)	0.998	0.328

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	293	1.5 M Lithium chloride/ 0.1 M BICINE 8.4 10 % w/v PEG 6000 7 mg/ml 0.2 / 0.2 drops