29ZE
Crystal structure of human NIF3L1 with monomer in ASU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 118.256, 118.256, 209.792 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.830 - 1.800 |
| R-factor | 0.1741 |
| Rwork | 0.174 |
| R-free | 0.18150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.961 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (("2.0_5936": ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.830 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 158800 | 1826 |
| <I/σ(I)> | 11.78 | 0.92 |
| Completeness [%] | 100.0 | 99.96 |
| Redundancy | 6.3 | |
| CC(1/2) | 0.999 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Sodium citrate tribasic dihydrate 0.1 M Tris 8.5 30 % v/v PEG 400 |
