29WM

Crystal structure of the G3BP1 NTF2L domain homodimer in complex with La Crosse virus nucleoprotein N-terminal peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.965
Spacegroup name	P 21 21 21
Unit cell lengths	83.099, 94.507, 113.331
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.580 - 2.400
R-factor	0.2309
Rwork	0.229
R-free	0.27750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.884
Data reduction software	XDS (2023-06-30)
Data scaling software	pointless (1.12.16)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.620	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.083	0.947
Rmeas	0.087	0.985
Rpim	0.024	0.270
Number of reflections	35528	3481
<I/σ(I)>	19.2	2.7
Completeness [%]	99.8	99.5
Redundancy	13	13.2
CC(1/2)	0.999	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.1 M MMT buffer (pH 9.0) and 25% PEG 1500