29TJ

Structure of the Porcine Reproductive and Respiratory Syndrome virus-2 glycoprotein GP4 antigenic region 59-69 bound to the neutralizing scFv#18

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 21 21 2
Unit cell lengths	72.283, 108.849, 50.156
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.550 - 2.300
R-factor	0.2036
Rwork	0.201
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.300	2.300
Number of reflections	18229	2893
<I/σ(I)>	8.22
Completeness [%]	100.0
Redundancy	13.3
CC(1/2)	0.997	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	20 % w/v Polyethylene glycol monomethyl ether 5,000, 200 mM lithium acetate