29QJ

Respiratory syncytial virus fusion protein N-terminal heptad repeat domain in complex with Double stapled peptide 4/4g

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	95
Detector technology	PIXEL
Collection date	2006-02-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	H 3 2
Unit cell lengths	41.083, 41.083, 200.844
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.520 - 1.325
R-factor	0.2062
Rwork	0.205
R-free	0.23210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.200
Data scaling software	Aimless (0.8.2)
Phasing software	PHASER (2.8.3)
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.000	1.432
High resolution limit [Å]	1.325	1.325
Rmerge	0.073	2.258
Rmeas	0.075	2.319
Rpim	0.017	0.522
Number of reflections	12173	609
<I/σ(I)>	18	1.2
Completeness [%]	77.3	19.3
Redundancy	19.8	19.5
CC(1/2)	0.997	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	20% PEG 3350 0.2M NH4I