29MQ
Sesterterpene Synthase from Streptomyces violarus (Sesterviolene Synthase, SvSS) in complex with the surrogate 2,3-dihydro-geranyl-farnesyl-pyrophosphate
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-08-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 |
| Unit cell lengths | 81.900, 98.210, 98.200 |
| Unit cell angles | 100.62, 113.36, 112.15 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| R-factor | 0.18033 |
| Rwork | 0.178 |
| R-free | 0.21919 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.223 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.062 | 0.749 |
| Number of reflections | 198778 | 28844 |
| <I/σ(I)> | 11.2 | 1.9 |
| Completeness [%] | 97.1 | 96.5 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 100 mM Tris, 10% PEG 8000 |
