29IM
LASV cap-snatching endonuclease in complex with BXA - SFX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE MFX |
| Synchrotron site | SLAC LCLS |
| Beamline | MFX |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2024-10-15 |
| Detector | SLAC ePix10k 2M |
| Wavelength(s) | 1.265 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 58.260, 58.260, 135.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.600 - 2.070 |
| R-factor | 0.2041 |
| Rwork | 0.201 |
| R-free | 0.23090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.921 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHENIX |
| Refinement software | PHENIX (2.0-5936_9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.040 | 2.100 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Number of reflections | 15394 | 762 |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 760.6 | |
| CC(1/2) | 0.968 | 0.801 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | After limited proteolysis with subtilisin A, 7.5 mg/ml target protein were mixed with an equal volume of crystallization solution (10% w/v PEG 10000, 250 mM MgSO4 and 100 mM Tris/HCl pH 9.0) |
