297D
X-RAY AND SOLUTION STUDIES OF DNA OLIGOMERS AND IMPLICATIONS FOR THE STRUCTURAL BASIS OF A-TRACT-DEPENDENT CURVATURE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.943, 40.544, 65.003 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.22 * |
| R-free | 0.21600 |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 0.040 * |
| Data reduction software | DENZO |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.500 | |
| Rmerge | 0.062 | |
| Number of reflections | 2427 | |
| Completeness [%] | 96.8 | 88 * |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE_HCL | ||
| 5 | 1 | 1 | NA CACODYLATE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 2 (mg/ml) | |
| 2 | 1 | drop | 12.5 (mM) | ||
| 3 | 1 | drop | spermine-4HCl | 1.2 (mM) | |
| 4 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 5 | 1 | drop | MPD | 10 (%(w/v)) | |
| 6 | 1 | reservoir | MPD | 30 (%(w/v)) |
