294D

INTERCALATED CYTOSINE MOTIF AND NOVEL ADENINE CLUSTERS IN THE CRYSTAL STRUCTURE OF TETRAHYMENA TELOMERE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1996-05-24
Detector	RIGAKU RAXIS II
Spacegroup name	P 2 21 21
Unit cell lengths	35.930, 52.330, 76.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.212
Rwork	0.212
R-free	0.29500
RMSD bond length	0.016
RMSD bond angle	3.740
Data reduction software	PROCESS
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.500	2.500 *
Rmerge	0.096
Total number of observations	33551 *
Number of reflections	4642
Completeness [%]	86.5	75 *
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5		pH 7.50, VAPOR DIFFUSION, SITTING DROP

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	WATER
2	1	1	NA CACODYLATE
3	1	2	WATER
4	1	2	NH4 SULFATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.0 (mM)
2	1	drop	sodium cacodylate	100 (mM)
3	1	reservoir	ammonium sulfate	70 (%)