293D
INTERACTION BETWEEN THE LEFT-HANDED Z-DNA AND POLYAMINE-2: THE CRYSTAL STRUCTURE OF THE D(CG)3 AND SPERMIDINE COMPLEX
Experimental procedure
| Temperature [K] | 283 |
| Detector technology | DIFFRACTOMETER |
| Detector | NICOLET P3 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 17.930, 31.230, 44.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 1.000 |
| R-factor | 0.191 |
| Starting model (for MR) | ZDF001 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | pH 7.00, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | SPERMIDINE_HCL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ammonium salt | 2 (mM) | |
| 2 | 1 | drop | d(CG)3 | ||
| 3 | 1 | drop | spermidine trichloride | 10 (mM) | |
| 4 | 1 | drop | 15 (mM) | ||
| 5 | 1 | drop | sodium cacodylate | 30 (mM) |
