28TM

Structure of an uncharacterized Rossmann fold protein from Homo sapiens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	35.863, 108.321, 122.356
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.550 - 2.920
R-factor	0.2552
Rwork	0.253
R-free	0.29950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.508
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (2.0_5936)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.550	3.210
High resolution limit [Å]	2.920	2.920
Number of reflections	19963	4991
<I/σ(I)>	4.62	1.1
Completeness [%]	93.2	74.9
Redundancy	6.9
CC(1/2)	0.992	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297.15	20% w/v PEG4K, 0.1 M CaCl2, 0.1 M Tris pH 8.5