28OJ

X-ray crystal structure of wild-type YdbL from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE VMXi
Synchrotron site	Diamond
Beamline	VMXi
Temperature [K]	293
Detector technology	PIXEL
Collection date	2024-05-10
Detector	DECTRIS EIGER2 X 4M
Wavelength(s)	0.7749
Spacegroup name	I 4 2 2
Unit cell lengths	63.994, 63.994, 95.851
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.220 - 2.120
R-factor	0.1996
Rwork	0.196
R-free	0.24040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.636
Data reduction software	xia2.multiplex
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.220	2.160
High resolution limit [Å]	2.120	2.120
Number of reflections	62429	3179
<I/σ(I)>	4.6
Completeness [%]	99.9
Redundancy	10.5
CC(1/2)	0.861	0.514

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES pH 7, 30 % (w/v) Jeffamine ED-200130 pH 7