28NM
Crystal structure of the human galectin-1 in complex with p-Methoxyphenyl 3-O-benzyl-beta-D-galactopyranoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-06-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.951, 57.627, 112.153 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.360 - 2.000 |
| R-factor | 0.23082 |
| Rwork | 0.228 |
| R-free | 0.27907 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 2.095 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.360 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 18083 | 1475 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 92.4 | |
| Redundancy | 7.3 | |
| CC(1/2) | 0.997 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200mM LiSO4, 100mM Tris-HCl pH 8.5, 35% PEG3350 |
