28KA

Iron loaded human H-chain ferritin, anaerobic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9999
Spacegroup name	F 4 3 2
Unit cell lengths	182.958, 182.958, 182.958
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.930 - 2.110
R-factor	0.1578
Rwork	0.154
R-free	0.19130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.817
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.930	2.181
High resolution limit [Å]	2.106	2.106
Number of reflections	31515	3063
<I/σ(I)>	56.52
Completeness [%]	99.9
Redundancy	2
CC(1/2)	1.000	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M bicine pH 9.0 0.1 M sodium chloride 2 M magnesium chloride 60 mM ferrous chloride 3 mM sodium azide