28IY
Crystal structure of the TPR domain of KLC1 in complex with the C-terminal peptide of JIP1 at 2.13 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.585, 89.545, 51.327 |
| Unit cell angles | 90.00, 100.56, 90.00 |
Refinement procedure
| Resolution | 68.340 - 2.129 |
| R-factor | 0.223 |
| Rwork | 0.222 |
| R-free | 0.24380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.890 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.340 | 2.260 |
| High resolution limit [Å] | 2.128 | 2.128 |
| Number of reflections | 21460 | 1074 |
| <I/σ(I)> | 12.7 | 1.3 |
| Completeness [%] | 92.9 | |
| Redundancy | 6.5 | 7 |
| CC(1/2) | 0.996 | 0.362 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M MMT buffer, 25% polyethylene glycol (PEG) 1500 |
