28EZ

Crystal structure of AvrSr50-like-0300 from Puccinia graminis f. sp. tritici

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95374
Spacegroup name	P 62 2 2
Unit cell lengths	108.921, 108.921, 83.767
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.160 - 2.180
R-factor	0.2229
Rwork	0.221
R-free	0.25270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.993
Data reduction software	XDS
Data scaling software	AutoProcess
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.160	2.310
High resolution limit [Å]	2.180	2.180
Number of reflections	15924	1320
<I/σ(I)>	19.9	2.1
Completeness [%]	99.9
Redundancy	23
CC(1/2)	0.999	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	277	0.1M Trisodium citrate, 22% (w/v) PEG 3350, 0.1M HEPES pH 7.2