28DN
CRYSTAL STRUCTURE ANALYSIS OF AN A(DNA) OCTAMER D(GTACGTAC)
Experimental procedure
| Temperature [K] | 279 |
| Detector technology | DIFFRACTOMETER |
| Detector | NICOLET |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.500, 42.500, 24.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.400 |
| R-factor | 0.17 |
| Rwork | 0.170 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.055 * |
| Total number of observations | 2682 * |
| Number of reflections | 894 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | pH 6.00, VAPOR DIFFUSION, SITTING DROP, temperature 293.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | SPERMINE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 2 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 25 (mM) | |
| 3 | 1 | drop | 15 (mM) | ||
| 4 | 1 | drop | spermine hydrochloride | 8 (mM) | |
| 5 | 1 | drop | MPD | 5 (%(v/v)) | |
| 6 | 1 | reservoir | MPD | 30 (%(v/v)) |
