28BS

Cryogenic temperature crystal structure of Nmar_1308 protein at 3.67 angstrom resolution

Replaces:  9ULB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 32
Unit cell lengths	78.450, 78.450, 207.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.210 - 3.670
R-factor	0.2565
Rwork	0.251
R-free	0.30620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.348
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.210	3.890
High resolution limit [Å]	3.670	3.670
Number of reflections	15594	2477
<I/σ(I)>	4.42
Completeness [%]	99.8
Redundancy	7.15
CC(1/2)	0.900	0.220

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		298	0.3M Sodium fluoride; 0.3M Sodium bromide, 0.3M Sodium iodide, 1.0M Tris (base), BICINE pH 8.5, 25% v/v MPD, 25% PEG 1000, 25% w/v PEG 3350