286D
X-RAY AND SOLUTION STUDIES OF DNA OLIGOMERS AND IMPLICATIONS FOR THE STRUCTURAL BASIS OF A-TRACT-DEPENDENT CURVATURE
Experimental procedure
| Source type | ROTATING ANODE |
| Temperature [K] | 120 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-08-01 |
| Detector | XENTRONICS |
| Spacegroup name | P 31 |
| Unit cell lengths | 33.240, 33.240, 45.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.203 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | B-DNA ORIENTED FIBER |
| RMSD bond length | 0.037 |
| RMSD bond angle | 0.070 |
| Data reduction software | XDS |
| Data scaling software | ULTIMA |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.500 | |
| Rmerge | 0.043 | |
| Number of reflections | 1928 * | |
| Completeness [%] | 96.9 | 88 * |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE_HCL | ||
| 5 | 1 | 1 | NA CACODYLATE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 2 (mg/ml) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | spermine-4HCl | 8 (mM) | |
| 4 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 5 | 1 | drop | MPD | 10 (%(w/v)) | |
| 6 | 1 | reservoir | MPD | 30 (%) |
