27XJ

Crystal Structure of Legionella pneumophila Glycosidase Effector LegY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	70.417, 79.112, 104.113
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.967 - 1.456
R-factor	0.1302
Rwork	0.130
R-free	0.15630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yep
RMSD bond length	0.014
RMSD bond angle	1.246
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	EPMR
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.967	1.510
High resolution limit [Å]	1.456	1.456
Number of reflections	97826	9929
<I/σ(I)>	20.82	3.2
Completeness [%]	94.9
Redundancy	10.7
CC(1/2)	0.999	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	20%PEG3350, 0.2 M Ammonium fluorid, 0.1 M Tris-HCl pH 6.6