27JP

Crystal structure of the mature form of human ASPRV1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	193
Detector technology	PIXEL
Collection date	2025-12-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	C 1 2 1
Unit cell lengths	260.139, 34.975, 185.550
Unit cell angles	90.00, 134.34, 90.00

Refinement procedure

Resolution	132.690 - 2.350
R-factor	0.2072
Rwork	0.204
R-free	0.26120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.066
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	132.690	2.420
High resolution limit [Å]	2.350	2.350
Number of reflections	50963	4328
<I/σ(I)>	12.4
Completeness [%]	99.7
Redundancy	5.9
CC(1/2)	0.998	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M magnesium formate dihydrate, pH 7.0, and 20% (v/v) PEG 3,350